Reporter

Part:BBa_K4813006:Design

Designed by: YuHin Lau   Group: iGEM23_HongKong-JSS   (2023-10-03)


J23100 - tdTomato red chromoprotein strong expression construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
    Illegal NheI site found at 50
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the presence of multiple high CG content regions in this particular part, it posed a challenge for direct synthesis by IDT. Despite optimization efforts, we still had to divide the sequence into three separate gBlock fragments for ordering. This approach allowed us to overcome the difficulties associated with the high CG content regions and proceed with the assembly process.


Source

The original tdTomato sequence was obtained from NCBI. Then, the sequence was codon optimized by IDT and gBlock was synthesized from IDT.

References